nigrum, a simple, accurate and precise HPLC-PDA method was developed for the simultaneous determination of the above-mentioned six compounds. The separation was achieved by Phenomenex Luna RP C-18 column (150 x 4.6 mm, 5 mu m, Phenomenex Inc, CA, USA) with a binary gradient solvent
system of water-acetonitrile, at a flow rate of 1.0 mLmin(-1) and detected at 210, 232, 262 and 343 nm. All six calibration curves showed good linearity (R-2 VX-680 datasheet > 0.9966). The method was reproducible with intra-and inter-day variations of less than 2% and 5%, respectively. The results demonstrated that this method is simple, reliable and suitable for the quality control of these plants.”
“Objective: To evaluate the pregnancy outcome in women > 30 years old with uterine fibroids also considering other possible concomitant risk factors. Methods: A multicentric, observational and retrospective study was conducted. All women were asked to complete a questionnaire
while they were in hospital and the outcome of pregnancy was investigated. Results: Women with uterine fibroids before pregnancy were more frequently > 35 years old and in their clinical history before pregnancy showed more pelvic pain, polliachiuria, previous pelvic surgery, hypermenorrhea Selleckchem BVD-523 and dysmenorrhea, than control women (p < 0.0001). During pregnancy patients with uterine fibroids showed significantly more threatened preterm birth (PTB) and PTB, weight gain, hyperemesis, gestational hypertension and post-partum bleeding and showed more often requirement for emergency
cesarean section (p < 0.0001). The multivariate logistic regression model used for evaluating the effect of clinical parameters on outcome of pregnancy showed that age > 35 years, pelvic pain, work, weight gain, hyperemesis, threatened miscarriage and threatened PTB were significantly correlated with unfavorable pregnancy outcome, independently of the presence of uterine fibroids (p < 0.0001). Conclusions: The present study confirming a more frequent negative pregnancy outcome in presence of uterine fibroids, showed that other risk factors may affect pregnancy and delivery outcome in women aging > 30 years old.”
“Activity-guided fractionations of leaf extracts from Laurus nobilis L. led to the isolation of a known sesquiterpene lactone, deacetyl laurenobiolide LGX818 supplier (1). Compound 1 showed antimicrobial activity against periopathic pathogens (Actinomyces viscosus, Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans), opportunistic Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes) and fungi (Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus). Furthermore, acetylation and cyclisation of deacetyl laurenobiolide (1) yielded laurenobiolide (2) and a new compound, (5S,6R,7S,8S,10R)-6,8-dihydroxyeudesma-4(15), 11(13)-dien-12-oic acid 12,8-lactone (3), respectively. Compounds 2 and 3 also showed antimicrobial activities.