, Sparks, MD) Middlebrook 7H11 selective agar supplemented with Polymyxin B (200,000 units), Carbenicillin (50.0 mg), Amphotericin B (10.0 mg), Trimethoprim Lactate (20.0 mg) to hinder bacterial and fungal overgrowth. CFU counts
were enumerated on day 14, 21 and day 28. Scoring of gross pathology A gross pathology scoring sheet was developed to enumerate the gross pathology seen at necropsy (Additional File 1). The sheet was based upon an earlier published scoring sheet in the cynomolgus maqaque model by Lin and colleagues [13]. Grossly visible lesions from all lung lobes and extrapulmonary sites were described and enumerated. The total score was determined by adding all subtotal numbers assigned to each evaluable anatomic site. Standard descriptive strategies were also employed to document disease burden at necropsy and compared to the developed OSI-027 molecular weight scoring system. Statistical analysis Data are reported as mean values unless otherwise stated. Mean quantitative
scores based on gross pathology were compared via non-parametric analyses by Mann-Whitney U test. Mean paired values of thoracic/extrapulmonary CFUs were summed and compared by paired t-test analyses. Tissue CFUs in each rabbit population were paired, regardless of sensitization status, during comparative tissue analyses. The level of significance was set at P < 0.10. Acknowledgements and Funding We gratefully acknowledge the support of NIH
grants/contracts Torin 2 AI36973, AI37856, AI079590, and AI30036. We thank Nicole C. Ammerman for her generous assistance in the acquisition of experimental data. Electronic supplementary material Additional file 1: Gross Scoring System Employed for the Rabbit of Tuberculosis. A scoring sheet was developed to enumerate the gross pathology seen at necropsy. Visible lesions from all lung lobes and extrapulmonary sites were described and enumerated (maximum possible score of 50). The total score was determined by adding Digestive enzyme all subtotal numbers assigned to each evaluable anatomic site. (DOCX 30 KB) References 1. World Health Organization: Global Eltanexor in vitro tuberculosis Control. Surveillance, Planning, Financing 2007. 2. Nardell EA, Piessens WF: Transmission of tuberculosis. In Tuberculosis: A comprehensive international approach. Edited by: LB Reichman, Hershfield ES. Marcel Dekker, New York (NY); 2000:215–240. 3. Iseman MD: A clinician’s guide to tuberculosis, Lippincott Williams & Wilkins, Philadelphia, PA. 2000, 51–62. 4. Kramnik I, Dietrich WF, Demant P, Bloom BR: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis. Proc Natl Acad Sci USA 2000, 97:8560–8565.PubMedCrossRef 5. Smith DW, Harding GE: Animal model: Experimenal airborne tuberculosis in the guinea pig. Am J Pathol 1977, 89:273–276.PubMed 6.