Colonies distinctly circular with well-defined margin, compact, hyaline, thin, silky, with fine concentric

zonation of unequal width. Hyphae radially arranged, thin, little on surface; surface hyphae degenerating, becoming multiguttulate. Aerial hyphae scant. Autolytic excretions rare; coilings variable, sometimes abundant. No distinct odour, no pigment noted. Chlamydospores uncommon. Conidiation noted after 4–6 days, better developed than on CMD, invisible to the unaided eye, effuse, on loosely disposed minute conidiophores spreading from the plug and proximal margin irregularly BKM120 across the entire colony; at the distal margin also verticillium-like on aerial hyphae. Conidial heads minute, <30 μm diam, wet, becoming dry, greenish in the stereo-microscope. Conidiophores (after 6–12 days at 25°C) to 150(–300) μm long, erect, simple, asymmetric, of a short stipe or single axis 3–5 μm wide, with a single terminal whorl of phialides and some scattered solitary phialides, or with up to five steep, unpaired main axes emerging at low levels. Main axes unbranched or with unpaired branches. Branches 2–3 μm wide at ends, bearing solitary phialides or

short, tree-like, often paired and mainly 1-celled terminal branches, strongly inclined upwards. Phialides arising from cells 2–4 μm wide, solitary or divergent in whorls of 2–4(–6). Phialides FK228 price (5–)7–12(–18) μm (n = 120) μm long, lageniform or subcylindrical, less commonly ampulliform with long neck, mostly inaequilateral.

Conidia as in granules. After ca 1 month (or growth for 16 days at 25°C plus 6–12 days at 15°C) Tacrolimus (FK506) conidiation becoming visible as minute, white to greenish granules or minipustules 0.2–0.8 mm diam, formed mainly along margin of the plate; slightly more complex and stout in structure than effuse conidiation. Compared to effuse conidiation, main axes more pachybasium-like, longer, with 1–2 fold branching at higher levels, terminal branches short, often paired and right-angled or inclined upwards, 1–3 celled. Branches 3–5(–6) μm wide. Phialides arising singly or in whorls on cells 2.5–4 μm wide. Phialides (4.5–)5.5–9.0(–12) × (2.3–)2.5–3.2(–3.7) μm, l/w (1.5–)1.7–3.2(–4.8), (1.4–)1.8–2.5(–2.8) μm (n = 61) wide at the base; narrowly lageniform or subulate, more rarely ampulliform, straight, sometimes curved or sinuous, usually widest below the middle, without conspicuous thickenings. Ampulliform phialides more frequent in microtufts or granules formed late. Phialides from simple conidiophores and granules combined (4.5–)6–11(–18) × (2.0–)2.5–3.3(–4.0) μm, l/w (1.5–)2–4(–7.5) (n = 181). Conidia (2.2–)2.5–3.5(–5.5) × (1.8–)2.0–2.5(–3.0) μm, l/w (1.0–)1.1–1.5(–2.1) (n = 180), subhyaline to pale yellowish green, subglobose, oval, less commonly ellipsoidal, smooth, with few minute SB202190 research buy guttules; scar indistinct. At 15°C growth irregular, effuse conidiation on the entire colony except the centre.