, 2007). Currently used surfactants are often petroleum derived, but production of these compounds from renewable substrates is now of considerable interest. Sophorolipids, which consist of the sugar sophorose linked to a long-chain hydroxy fatty acid, are among candidate compounds that can be produced from renewable sources. Interest in sophorolipids is not limited to KU-60019 cost production of surfactants. The unique chemical structure of sophorolipids can serve as the basis for synthesizing certain hydroxy fatty acids and other compounds (Van Bogaert et al., 2007). Perhaps of greater interest are reports that these glycolipids have antimicrobial activity against certain yeasts (Ito et al., 1980), plant pathogenic fungi (Yoo et

al., 2005) and bacteria (Mager et al.,

1987; Lang et al., 1989). Furthermore, Shah et al. (2005) showed inhibition of the HIV virus by sophorolipids, and Chen et al. (2006) provided evidence that the compounds have anticancer activity. Sophorolipids Aloxistatin nmr are synthesized by a phylogenetically diverse group of yeasts. The earliest report appears to be that of Gorin et al. (1961), who demonstrated sophorolipid biosynthesis by the anamorphic ascomycetous yeast Candida apicola, which was initially identified as Candida magnoliae. Later, Spencer et al. (1970) showed sophorolipid production by Candida bombicola, and Konoshi et al. (2008) reported Candida batistae to also form sophorolipids. The preceding MRIP three Candida sp. are closely related, but sophorolipid biosynthesis was also demonstrated for the less closely related Wickerhamiella domercqiae (Chen et al., 2006) as well as for the basidiomycetous yeast Rhodotorula bogoriensis (Tulloch et al., 1968). Phylogenetic analysis of sequences for the D1/D2 domains of the nuclear large subunit (LSU) rRNA gene has shown that C. apicola and C. bombicola are members of a clade that is well separated from other ascomycetous

yeasts (Kurtzman & Robnett, 1998). Candida bombicola is the first member of the clade for which ascospore formation was discovered and the species was reassigned to the teleomorphic genus Starmerella (Rosa & Lachance, 1998). With the application of sequence analysis to yeast identification, the group of yeasts related to Starmerella bombicola, now termed the Starmerella clade, has increased markedly in the past decade to over 40 species. Many of these species have not been described as yet but are presently recognized from their gene sequences, which have been deposited in GenBank. Candida apicola, C. batistae and S. bombicola are the only members of the Starmerella clade that have been reported to produce sophorolipids. In the present work, we examined additional species of the Starmerella clade for production of sophorolipids using a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) MS-based screen similar to that used previously to identify bacterial biosurfactants, rhamnolipids, surfactins and iturins (Price et al.