Chronic HBV infection develops in 90 %of newborns, 29-40 %of children and 5-10 %of adults who were infected. Several studies have reported that the BCP/ PC mutants may be associated with progression of fulminant hepatic failure. However, virologic

and clinical features of children patients with CHB and LC have not been well documented. This study is to investigate virologic and clinical characters of basal core promoter (BCP)and precore (PC) region mutations in children with chronic hepatitis B and hepatitis B related liver cirrosis. Methods: A total of 307 patients with a CHB selleck screening library infection, including 88 with hepatitis B related liver cirrhosis and 219 with chronic hepatitis B were enrolled. The HBV genotypes and the presence of mutations in the BCP/PC regions were determined by direct sequencing. Biochemical and serological parameters as well as HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested

PF01367338 PCR. Genotypes/subgenotypes were determined by derect DNA sequencing followed by molecular evolutionary analysis of the viral sequences. Mutations at 11 interested sites of the BCP/PC region were compared among the two groups of patients. Results: 46/307 (14.98%) were infected with genotype B and 261/307 (85.02%) with genotype C. LC and CHB patients both had a significantly higher ratio of genotype C to B (81.9%-18.1 %vs. 70.1%-29.9 %).The prevalence of BCP/PC wild-type virus was 54.3 %in CHB patients in contrast to 4.8 %in LC patients. In genotype C patients, the C1653T T1753C, A1762T, G1764A, G1896A mutations were significantly higher prevalent in LC patients.

Genotype B virus had higher 1752 mutation frequency. Genotype C virus had higher prevalence of T1753C, T1758C, A1762T, G1764A, G1896A mutation frequency compared to genotype find more B virus. CHB patients with BCP/PC mutant virus had higher viral load, whereas LC patients with BCP/PC mutant virus had higher viral load and elevated alanine aminotransferase in comparison with those with the wild-type virus. Conclusions: Children patients with genotype C virus, BCP/PC C1653T, A1762T, G1764A, G1896A mutant virus were more susceptible to develop LC, whereas high prevalence of the BCP/PC mutations was associated with CHB development. Disclosures: The following people have nothing to disclose: Yanwei Zhong, Hongfei Zhang, Shishu Zhu, Yi Dong, Zhiqiang Xu, Dawei Chen, Hui Dong, Fenglin Di, Limin Wang, Yu Gan, Fuchuan Wang.