Methods: Collect

33 cases of Kazak esophageal squamous cell carcinoma and 38 cases of local normal esophageal tissue, and 32 cases of Han nationality esophageal squamous cell carcinoma and 34 cases of local normal esophageal tissue, useing MassARRAY methylation DNA quantitative analysis technology to detect the methylation status of smad4 gene promoter. Results: ① The average methylation rate of smad4 gene promoter CpG units were 3.44% in Han nationality PD0325901 ic50 esophageal cancer and 3.18% in control groups, the average methylation rate of smad4 gene promoter CpG units were 3.41% in Kazak esophageal cancer and 2.51% in control groups, the difference was not statistically significant (P > 0.05). ② The average methylation rate of smad4 gene in Han nationality esophageal CpG units 15 (4.75%) is significantly PF-02341066 manufacturer higher than the control group (3.62%); The average methylation rate of smad4 gene in Kazak esophageal CpG units 1, CpG units 16–19, units 27–28, units 31–33 (1.66%, 4.34%, 4.81%, 6.81%) were

signific- antly higher than the control group (0.72%, 2.24%, 3.06%, 5.51%), the average methylation rate of CpG units 6 in Kazak esophageal cancer (1.84%) is significantly higher than Han nationality cancer (0.44%); The average methylation rate of CpG units 14, units 16 between Kazak (6.51%, 4.34%) and Han nationality (6.87%, 4.03%) normal tissue were difference; the average methylation rate of CpG units 6, units 15, units 16–19, units 27–28, units 31–33 between Kazak (0.011%, 0.031%, 0.022%, 0.030%, 0.055%) and Han nationality (0.004%, 0.048%, 0.040%, 0.049%, 0.078%) normal tissue were difference; the difference was statistically significant (P < 0.05). Conclusion: ① Smad4 gene promoter hypermethylation was Participate in esophageal cancer both in Kazak esophageal cancer and Han nationality

esophageal cancer and may be used as diagnostic markers. ② Smad4 gene promoter hypermethylation in CpG Unit 15 may connected with the Kazakh esophageal cancer. Hypermethylation in CpG units selleck chemicals 1, units 16–19, units 27–28, units 31–33 may be the early events and connected with the Kazakh esophageal cancer. Smad4 gene promoter hypermethylation in CpG Unit 6, Unit 16–19 may the reason that High incidence of Kazakh esophageal cancer than Han nationality esophageal cancer. Key Word(s): 1. Han nationality; 2. Kazak; 3. smad4 gene; 4. esophageal cancer; Presenting Author: QINGXIANG YU Additional Authors: BANGMAO WANG Corresponding Author: BANGMAO WANG Affiliations: qingxiang.yu@qq.